Detailed information of TA system TA028619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1117394..1118012
• Replicon: chromosome
• Accession: NC_019845
• Organism: Sinorhizobium meliloti GR4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: H0FWR0
• Locus tag: C770_RS05235
• Protein ID: WP_003527306.1
• Coordinates: 1117716..1118012 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C770_RS05230
• Protein ID: WP_010969010.1
• Coordinates: 1117394..1117702 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C770_RS05185	1112472..1112792	-	321	WP_003527320.1	YnfA family protein	-
C770_RS50115	1112868..1113041	-	174	WP_010969006.1	hypothetical protein	-
C770_RS50120	1113055..1113228	-	174	WP_015241093.1	hypothetical protein	-
C770_RS05200	1113464..1114165	+	702	WP_014529127.1	glutathione S-transferase N-terminal domain-containing protein	-
C770_RS05205	1114389..1114730	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
C770_RS05210	1115315..1115581	+	267	WP_015241094.1	sel1 repeat family protein	-
C770_RS05215	1115603..1116724	-	1122	WP_015241095.1	pyridoxal phosphate-dependent aminotransferase	-
C770_RS05225	1117152..1117385	-	234	WP_003527308.1	hypothetical protein	-
C770_RS05230	1117394..1117702	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
C770_RS05235	1117716..1118012	-	297	WP_003527306.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C770_RS05240	1118060..1118365	-	306	WP_003527305.1	hypothetical protein	-
C770_RS05245	1118693..1119736	+	1044	WP_015241096.1	porin	-
C770_RS05250	1119846..1120912	-	1067	Protein_1036	site-specific integrase	-
C770_RS05255	1120953..1122002	-	1050	WP_015241098.1	porin	-
C770_RS05260	1122216..1122704	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11601.01 Da
• Isoelectric Point: 6.7262

>T28619 WP_003527306.1 NC_019845:c1118012-1117716 [Sinorhizobium meliloti GR4]
VIVGFRDDWLRTFFVDDVRSRNIPSDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T28619 NC_019845:c1118012-1117716 [Sinorhizobium meliloti GR4]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTCCGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACAGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT28619 WP_010969010.1 NC_019845:c1117702-1117394 [Sinorhizobium meliloti GR4]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT28619 NC_019845:c1117702-1117394 [Sinorhizobium meliloti GR4]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: H0FWR0