Detailed information of TA system TA028614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 409614..410297
• Replicon: plasmid pDEIPE01
• Accession: NC_019789
• Organism: Deinococcus peraridilitoris DSM 19664

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: DEIPE_RS20675
• Protein ID: WP_041231956.1
• Coordinates: 409614..409976 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: L0A903
• Locus tag: DEIPE_RS20680
• Protein ID: WP_015231498.1
• Coordinates: 409980..410297 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEIPE_RS20650	405550..405729	-	180	WP_041231826.1	hypothetical protein	-
DEIPE_RS20655	405972..406415	-	444	WP_015231494.1	DMT family transporter	-
DEIPE_RS23470	406637..406831	+	195	WP_083865921.1	hypothetical protein	-
DEIPE_RS20660	406887..407171	+	285	WP_041231827.1	winged helix-turn-helix domain-containing protein	-
DEIPE_RS20665	407265..407672	+	408	WP_015231495.1	RidA family protein	-
DEIPE_RS20670	407678..408676	+	999	WP_015231496.1	threonine/serine dehydratase	-
DEIPE_RS20675	409614..409976	+	363	WP_041231956.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DEIPE_RS20680	409980..410297	+	318	WP_015231498.1	XRE family transcriptional regulator	Antitoxin
DEIPE_RS20685	410456..411310	-	855	WP_015231499.1	IS256 family transposase	-
DEIPE_RS23475	411403..411876	+	474	WP_015231500.1	hypothetical protein	-
DEIPE_RS23480	411840..412588	-	749	Protein_405	IS1 family transposase	-
DEIPE_RS20705	412712..412993	+	282	WP_015231501.1	HNH endonuclease	-
DEIPE_RS20710	413028..414653	+	1626	WP_015231502.1	MBL fold metallo-hydrolase	-
DEIPE_RS24275	414687..414830	+	144	WP_157449120.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..556630	556630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-92)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13484.31 Da
• Isoelectric Point: 4.3553

>T28614 WP_041231956.1 NC_019789:409614-409976 [Deinococcus peraridilitoris DSM 19664]
ILCVTWTIILLDEVTAWFDSLDDETTQLVTSAIDMLEERGPSLGRPMVDTLEGTNLPNLKELCPGSSGRSEIRILFVAPG
ILLTAGDKTGHWKRWYKEHIPIAEAHCHEGLKDDRDQEEL

Nucleotide

• Length: 363 bp

>T28614 NC_019789:409614-409976 [Deinococcus peraridilitoris DSM 19664]
ATTTTATGCGTAACGTGGACGATCATTCTTCTGGACGAAGTCACCGCCTGGTTCGACAGCCTCGATGATGAAACGACTCA
ACTGGTTACCAGCGCCATTGACATGCTCGAAGAACGCGGCCCAAGCCTTGGACGCCCCATGGTTGACACTCTCGAAGGAA
CCAACCTGCCGAACCTCAAAGAGCTTTGTCCCGGTTCCAGTGGCCGCAGTGAAATCCGCATTCTTTTCGTAGCGCCGGGC
ATCCTCCTGACGGCTGGCGACAAGACAGGACATTGGAAGCGGTGGTACAAGGAACATATTCCCATCGCTGAGGCCCATTG
CCACGAAGGGCTGAAAGACGATCGCGACCAAGAGGAGTTGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11729.35 Da
• Isoelectric Point: 9.8559

>AT28614 WP_015231498.1 NC_019789:409980-410297 [Deinococcus peraridilitoris DSM 19664]
MPKTWKQVRAEAAERGRIDEAKVAEHREAGLASVRAYRLAELRKRTGFNQEEIAQKVGVTQSRVSRIERGDIGHTELGTL
RAFTEAFGGELEVVVKLGDERVVLV

Nucleotide

• Length: 318 bp

>AT28614 NC_019789:409980-410297 [Deinococcus peraridilitoris DSM 19664]
ATGCCGAAGACTTGGAAGCAAGTTCGTGCCGAAGCTGCCGAACGCGGACGTATTGACGAGGCGAAGGTTGCTGAGCACCG
CGAAGCCGGACTCGCCAGTGTGCGCGCATACCGCCTCGCAGAACTCCGCAAGAGGACTGGATTTAACCAAGAGGAAATCG
CGCAGAAGGTCGGCGTCACGCAATCACGGGTTTCCCGCATCGAACGAGGTGACATCGGGCACACTGAACTCGGCACCCTC
CGCGCGTTCACAGAAGCATTTGGCGGTGAGCTTGAAGTGGTCGTCAAGTTGGGCGATGAGCGCGTCGTGCTCGTCTGA

Structures

Antitoxin

• AlphaFold DB: L0A903