Detailed information of TA system TA028598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5105862..5106484
• Replicon: chromosome
• Accession: NC_019771
• Organism: Anabaena cylindrica PCC 7122

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K9ZM56
• Locus tag: ANACY_RS22305
• Protein ID: WP_015216485.1
• Coordinates: 5106185..5106484 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ANACY_RS22300
• Protein ID: WP_015216484.1
• Coordinates: 5105862..5106173 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ANACY_RS22280	5101605..5102492	-	888	WP_042465302.1	sugar ABC transporter permease	-
ANACY_RS22285	5102529..5103206	+	678	WP_015216481.1	phosphoribosylglycinamide formyltransferase	-
ANACY_RS22290	5103488..5104930	+	1443	WP_015216482.1	phosphoglucomutase/phosphomannomutase family protein	-
ANACY_RS22295	5105010..5105588	+	579	WP_015216483.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
ANACY_RS22300	5105862..5106173	-	312	WP_015216484.1	HigA family addiction module antidote protein	Antitoxin
ANACY_RS22305	5106185..5106484	-	300	WP_015216485.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ANACY_RS22310	5106661..5106999	-	339	WP_015216486.1	P-II family nitrogen regulator	-
ANACY_RS22315	5107139..5109625	-	2487	WP_015216487.1	CHAT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-66)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11944.37 Da
• Isoelectric Point: 5.6761

>T28598 WP_015216485.1 NC_019771:c5106484-5106185 [Anabaena cylindrica PCC 7122]
MSPSENYPKYRDKRTEQFALGERVKEFQAFTRQAYRRLEILEAATSKKDLMLLPSNHFESLEGDRDGQYSIHINEKWRIC
FNWPDDELKPFNIEITDYH

Nucleotide

• Length: 300 bp

>T28598 NC_019771:c5106484-5106185 [Anabaena cylindrica PCC 7122]
ATGTCTCCGTCAGAAAATTATCCAAAATATCGAGATAAAAGGACGGAGCAATTCGCTTTAGGTGAGAGAGTAAAAGAGTT
TCAAGCTTTTACTAGACAAGCATACAGACGGCTTGAAATATTAGAGGCGGCTACTAGTAAAAAGGATTTAATGTTATTAC
CAAGTAATCATTTTGAGTCCTTAGAAGGTGATAGAGACGGCCAATACAGCATCCACATTAATGAAAAATGGCGAATTTGT
TTCAATTGGCCAGATGATGAACTAAAACCTTTCAACATAGAAATTACAGATTATCACTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11547.32 Da
• Isoelectric Point: 6.7259

>AT28598 WP_015216484.1 NC_019771:c5106173-5105862 [Anabaena cylindrica PCC 7122]
MARPPIHPGEILADELHELGISASELARLLHVPKNRITEIINGQRSITADTALRLGQWFGTSAEFWMNLQKNYELRLAEQ
KIGKEIKATISPWISTGDMQVLA

Nucleotide

• Length: 312 bp

>AT28598 NC_019771:c5106173-5105862 [Anabaena cylindrica PCC 7122]
ATGGCACGTCCACCTATTCATCCTGGAGAAATTTTAGCTGATGAACTGCATGAATTAGGAATAAGTGCAAGTGAATTAGC
TAGGTTGCTTCATGTCCCAAAAAATAGAATTACCGAAATAATTAATGGTCAAAGGTCAATTACTGCTGACACTGCATTAC
GTCTAGGACAATGGTTTGGTACTAGTGCTGAATTTTGGATGAATTTGCAAAAAAATTATGAACTCAGACTGGCTGAACAA
AAGATTGGTAAAGAAATTAAAGCAACCATTTCTCCTTGGATATCAACTGGGGATATGCAAGTTTTAGCCTAA

Structures

Toxin

• AlphaFold DB: K9ZM56