Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2277489..2278408 Replicon chromosome
Accession NZ_LR027516
Organism Mycobacterium tuberculosis strain DKC2 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag DKC2_RS11075 Protein ID WP_003900449.1
Coordinates 2277803..2278408 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag DKC2_RS11070 Protein ID WP_003410124.1
Coordinates 2277489..2277794 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKC2_RS11035 2272500..2273756 - 1257 WP_003410095.1 HNH endonuclease -
DKC2_RS11045 2274110..2274685 + 576 WP_003410100.1 hypothetical protein -
DKC2_RS11050 2274682..2275722 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
DKC2_RS11055 2275964..2276683 + 720 WP_003410108.1 DUF433 domain-containing protein -
DKC2_RS11060 2276673..2277089 + 417 WP_003410114.1 hypothetical protein -
DKC2_RS11065 2277105..2277404 - 300 WP_003410120.1 hypothetical protein -
DKC2_RS11070 2277489..2277794 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
DKC2_RS11075 2277803..2278408 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DKC2_RS11080 2278433..2278792 - 360 WP_003410131.1 hypothetical protein -
DKC2_RS11085 2278952..2279410 - 459 Protein_2095 hypothetical protein -
DKC2_RS11090 2279407..2280924 - 1518 Protein_2096 DEAD/DEAH box helicase -
DKC2_RS11095 2281434..2282432 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T285959 WP_003900449.1 NZ_LR027516:c2278408-2277803 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT285959 WP_003410124.1 NZ_LR027516:c2277794-2277489 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References