Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2212402..2213270 Replicon chromosome
Accession NZ_LR027516
Organism Mycobacterium tuberculosis strain DKC2 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag DKC2_RS10675 Protein ID WP_010886136.1
Coordinates 2212402..2212779 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag DKC2_RS10680 Protein ID WP_003409886.1
Coordinates 2212821..2213270 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKC2_RS10625 2208191..2208643 - 453 WP_003899095.1 lipoprotein -
DKC2_RS10630 2208707..2209108 + 402 WP_003409869.1 hypothetical protein -
DKC2_RS10635 2209101..2209283 - 183 WP_003409870.1 hypothetical protein -
DKC2_RS10640 2209397..2209747 - 351 WP_003899096.1 hypothetical protein -
DKC2_RS10645 2209758..2210660 - 903 WP_003899097.1 hypothetical protein -
DKC2_RS10650 2210681..2210872 - 192 WP_003409876.1 hypothetical protein -
DKC2_RS10655 2210873..2211169 - 297 WP_003409877.1 hypothetical protein -
DKC2_RS10660 2211409..2211624 + 216 WP_003409878.1 antitoxin -
DKC2_RS10665 2211621..2211932 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
DKC2_RS21630 2211906..2212427 - 522 WP_003904745.1 hypothetical protein -
DKC2_RS10675 2212402..2212779 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
DKC2_RS10680 2212821..2213270 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
DKC2_RS10685 2213267..2213812 + 546 WP_003409891.1 SecB-like chaperone -
DKC2_RS10690 2213701..2214315 - 615 WP_003901296.1 hypothetical protein -
DKC2_RS10695 2214364..2214660 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
DKC2_RS10700 2214657..2214908 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
DKC2_RS10705 2214895..2215389 + 495 WP_003899099.1 hypothetical protein -
DKC2_RS10710 2215549..2215956 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
DKC2_RS10715 2215960..2216232 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
DKC2_RS10720 2216265..2217485 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T285953 WP_010886136.1 NZ_LR027516:2212402-2212779 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT285953 WP_003409886.1 NZ_LR027516:2212821-2213270 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References