Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4397193..4397920 Replicon chromosome
Accession NZ_LR025101
Organism Escherichia coli isolate EC-1639

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag EC1639_RS21190 Protein ID WP_000550189.1
Coordinates 4397606..4397920 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag EC1639_RS21185 Protein ID WP_000560253.1
Coordinates 4397193..4397609 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1639_RS21175 4392608..4394959 + 2352 WP_000695464.1 alpha-glucosidase -
EC1639_RS21180 4395130..4397148 + 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
EC1639_RS21185 4397193..4397609 - 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
EC1639_RS21190 4397606..4397920 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
EC1639_RS21195 4398205..4399341 - 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
EC1639_RS21200 4399426..4399929 + 504 WP_001309773.1 M48 family metallopeptidase -
EC1639_RS21205 4400006..4400689 + 684 WP_001183054.1 vancomycin high temperature exclusion protein -
EC1639_RS21210 4400768..4401754 + 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T285911 WP_000550189.1 NZ_LR025101:c4397920-4397606 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT285911 WP_000560253.1 NZ_LR025101:c4397609-4397193 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References