Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2502117..2502679 Replicon chromosome
Accession NZ_LR025101
Organism Escherichia coli isolate EC-1639

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag EC1639_RS12145 Protein ID WP_000605676.1
Coordinates 2502401..2502679 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag EC1639_RS12140 Protein ID WP_000781370.1
Coordinates 2502117..2502401 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1639_RS12125 2497299..2500346 + 3048 WP_077577597.1 formate dehydrogenase-N subunit alpha -
EC1639_RS12130 2500359..2501243 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
EC1639_RS12135 2501236..2501889 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
EC1639_RS12140 2502117..2502401 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
EC1639_RS12145 2502401..2502679 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EC1639_RS12150 2502865..2503875 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
EC1639_RS12155 2504009..2505706 - 1698 WP_000433464.1 malate dehydrogenase -
EC1639_RS12160 2505863..2506000 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
EC1639_RS12165 2506102..2506317 - 216 WP_000495766.1 biofilm-dependent modulation protein -
EC1639_RS12170 2506662..2507093 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T285902 WP_000605676.1 NZ_LR025101:c2502679-2502401 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT285902 WP_000781370.1 NZ_LR025101:c2502401-2502117 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References