Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1095844..1096538 Replicon chromosome
Accession NZ_LR025101
Organism Escherichia coli isolate EC-1639

Toxin (Protein)


Gene name yafO Uniprot ID V0YKG6
Locus tag EC1639_RS05160 Protein ID WP_001263485.1
Coordinates 1096140..1096538 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EC1639_RS05155 Protein ID WP_000554758.1
Coordinates 1095844..1096137 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1639_RS05135 1091261..1091758 + 498 WP_000006263.1 REP-associated tyrosine transposase RayT -
EC1639_RS05140 1092197..1093909 - 1713 Protein_964 flagellar biosynthesis protein FlhA -
EC1639_RS05145 1093881..1094666 + 786 WP_000207554.1 putative lateral flagellar export/assembly protein LafU -
EC1639_RS05150 1094737..1095792 + 1056 WP_001226177.1 DNA polymerase IV -
EC1639_RS05155 1095844..1096137 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EC1639_RS05160 1096140..1096538 + 399 WP_001263485.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EC1639_RS05165 1096548..1097000 + 453 WP_001059899.1 GNAT family N-acetyltransferase -
EC1639_RS05170 1097246..1097452 + 207 Protein_970 RtcB family protein -
EC1639_RS26030 1097448..1097800 + 353 Protein_971 peptide chain release factor H -
EC1639_RS05180 1097857..1099314 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
EC1639_RS05185 1099575..1100033 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
EC1639_RS05190 1100125..1101369 + 1245 WP_001586695.1 esterase FrsA -
- 1100629..1100709 + 81 NuclAT_12 - -
- 1100629..1100709 + 81 NuclAT_12 - -
- 1100629..1100709 + 81 NuclAT_12 - -
- 1100629..1100709 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15418.84 Da        Isoelectric Point: 7.3840

>T285889 WP_001263485.1 NZ_LR025101:1096140-1096538 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT285889 WP_000554758.1 NZ_LR025101:1095844-1096137 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YKG6

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References