Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1062316..1062857 Replicon chromosome
Accession NZ_LR025096
Organism Escherichia coli isolate EC-7215

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EC7215_RS05000 Protein ID WP_000615976.1
Coordinates 1062316..1062594 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EC7215_RS05005 Protein ID WP_000729704.1
Coordinates 1062597..1062857 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC7215_RS04985 1059898..1060476 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EC7215_RS04990 1060682..1061449 + 768 WP_000333380.1 class II glutamine amidotransferase -
EC7215_RS04995 1061420..1062160 - 741 WP_001225679.1 murein L,D-transpeptidase -
EC7215_RS05000 1062316..1062594 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EC7215_RS05005 1062597..1062857 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EC7215_RS05010 1063043..1063816 + 774 WP_000543895.1 C40 family peptidase -
EC7215_RS05015 1063873..1064229 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
EC7215_RS05020 1064222..1064500 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EC7215_RS05025 1064605..1066317 - 1713 Protein_956 flagellar biosynthesis protein FlhA -
EC7215_RS05030 1066289..1067074 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1053367..1064500 11133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T285852 WP_000615976.1 NZ_LR025096:c1062594-1062316 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT285852 WP_000729704.1 NZ_LR025096:c1062857-1062597 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References