285849

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 489923..490420 Replicon chromosome
Accession NZ_LR025096
Organism Escherichia coli isolate EC-7215

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EC7215_RS02270 Protein ID WP_001173343.1
Coordinates 490247..490420 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EC7215_RS02265 Protein ID WP_000398619.1
Coordinates 489923..490219 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC7215_RS02245 486654..487373 - 720 WP_000611288.1 two-component system response regulator DcuR -
EC7215_RS02250 487370..489001 - 1632 WP_134159155.1 sensor histidine kinase -
EC7215_RS02255 489182..489412 + 231 WP_000371699.1 (4Fe-4S)-binding protein -
EC7215_RS02260 489424..489696 + 273 WP_000405647.1 N-acetyltransferase -
EC7215_RS02265 489923..490219 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EC7215_RS02270 490247..490420 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EC7215_RS02275 490539..492056 - 1518 WP_001295063.1 lysine--tRNA ligase -
EC7215_RS02280 492293..493750 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 489923..499273 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T285849 WP_001173343.1 NZ_LR025096:490247-490420 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT285849 WP_000398619.1 NZ_LR025096:489923-490219 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References