Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1740113..1740706 Replicon chromosome
Accession NZ_LN885086
Organism Candidatus Nitrospira inopinata isolate ENR4

Toxin (Protein)


Gene name higB Uniprot ID A0A0S4KUC7
Locus tag NITINOP_RS08365 Protein ID WP_062484746.1
Coordinates 1740113..1740403 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S4KTZ4
Locus tag NITINOP_RS08370 Protein ID WP_062487892.1
Coordinates 1740440..1740706 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NITINOP_RS08345 1735295..1736053 - 759 WP_062484742.1 hypothetical protein -
NITINOP_RS08350 1736250..1738733 + 2484 WP_158023303.1 ATP-dependent DNA helicase RecG -
NITINOP_RS08355 1738795..1739118 + 324 WP_062484744.1 hypothetical protein -
NITINOP_RS08360 1739115..1740104 + 990 WP_062484745.1 Ppx/GppA family phosphatase -
NITINOP_RS08365 1740113..1740403 + 291 WP_062484746.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
NITINOP_RS08370 1740440..1740706 + 267 WP_062487892.1 HigA family addiction module antidote protein Antitoxin
NITINOP_RS16140 1740728..1740883 + 156 WP_158023494.1 hypothetical protein -
NITINOP_RS08375 1741367..1742857 - 1491 WP_062484747.1 class I SAM-dependent methyltransferase -
NITINOP_RS08380 1742921..1743298 - 378 WP_158023304.1 hypothetical protein -
NITINOP_RS08390 1743598..1744065 + 468 WP_062484750.1 hypothetical protein -
NITINOP_RS08395 1744394..1745410 + 1017 WP_162264704.1 pentapeptide repeat-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-66)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11389.85 Da        Isoelectric Point: 6.7274

>T285755 WP_062484746.1 NZ_LN885086:1740113-1740403 [Candidatus Nitrospira inopinata]
VIESFANRLAEDLFDDRQSKAVLKFPSDLIRTARRKLLYLHDASEIGDLREPPGNRLEALKGEWKGFYSIRINDQWRVVF
RWHDGNAYEVQIIDYH

Download         Length: 291 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10061.02 Da        Isoelectric Point: 11.2862

>AT285755 WP_062487892.1 NZ_LN885086:1740440-1740706 [Candidatus Nitrospira inopinata]
MRLPKNPFHPGEMLLEEFLIPRKMTQAALAKKLGWTRARLNELIKGKRDITADSALDLARVLGTSAKLWMNLQATFDLDK
AMRKRKIA

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S4KUC7

Antitoxin

Source ID Structure
AlphaFold DB A0A0S4KTZ4

References