Detailed information of TA system TA285674

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4575973..4576385
• Replicon: chromosome
• Accession: NZ_LN832404
• Organism: Escherichia coli K-12 substr. AG100

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: EK12AG100_RS22660
• Protein ID: WP_000132601.1
• Coordinates: 4575973..4576314 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4576309..4576385 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EK12AG100_RS22650	4573386..4574432	-	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
EK12AG100_RS22655	4574432..4575811	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
EK12AG100_RS22660	4575973..4576314	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
-	4576309..4576385	+	77	NuclAT_13	-	Antitoxin
-	4576309..4576385	+	77	NuclAT_13	-	Antitoxin
-	4576309..4576385	+	77	NuclAT_13	-	Antitoxin
-	4576309..4576385	+	77	NuclAT_13	-	Antitoxin
-	4576309..4576385	+	77	NuclAT_14	-	Antitoxin
-	4576309..4576385	+	77	NuclAT_14	-	Antitoxin
-	4576309..4576385	+	77	NuclAT_14	-	Antitoxin
-	4576309..4576385	+	77	NuclAT_14	-	Antitoxin
EK12AG100_RS22665	4576542..4577936	-	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
EK12AG100_RS22670	4577933..4579522	-	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4568888..4577936	9048

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T285674 WP_000132601.1 NZ_LN832404:c4576314-4575973 [Escherichia coli K-12]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

Antitoxin

Nucleotide

• Length: 77 bp

>AT285674 NZ_LN832404:4576309-4576385 [Escherichia coli K-12]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin