Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2092147..2092649 Replicon chromosome
Accession NZ_LN832404
Organism Escherichia coli K-12 substr. AG100

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EK12AG100_RS10595 Protein ID WP_000767829.1
Coordinates 2092147..2092401 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EK12AG100_RS10600 Protein ID WP_001259255.1
Coordinates 2092398..2092649 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EK12AG100_RS10570 2087162..2087389 - 228 WP_000234896.1 sulfurtransferase TusA -
EK12AG100_RS10575 2087403..2088461 - 1059 WP_000492339.1 transport protein YeeE -
EK12AG100_RS10580 2088640..2089998 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EK12AG100_RS25690 2089988..2090050 - 63 WP_010723108.1 membrane protein YoeI -
EK12AG100_RS10585 2090265..2091194 - 930 WP_000803351.1 LysR family transcriptional regulator -
EK12AG100_RS10590 2091240..2092064 - 825 WP_000754737.1 SDR family oxidoreductase -
EK12AG100_RS10595 2092147..2092401 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EK12AG100_RS10600 2092398..2092649 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EK12AG100_RS24025 2092932..2092982 + 51 WP_001364200.1 his operon leader peptide -
EK12AG100_RS10605 2093128..2094027 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
EK12AG100_RS10610 2094033..2095337 + 1305 WP_000009594.1 histidinol dehydrogenase -
EK12AG100_RS10615 2095334..2096404 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
EK12AG100_RS10620 2096404..2097471 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T285663 WP_000767829.1 NZ_LN832404:c2092401-2092147 [Escherichia coli K-12]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT285663 WP_001259255.1 NZ_LN832404:c2092649-2092398 [Escherichia coli K-12]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References