Detailed information of TA system TA285589

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63302..63728
• Replicon: plasmid E
• Accession: NZ_LN824138
• Organism: Klebsiella pneumoniae strain MS6671

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: MS6671_RS31545
• Protein ID: WP_001312861.1
• Coordinates: 63302..63460 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	63504..63728 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6671_RS29350	58325..58552	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
MS6671_RS31525	58689..59360	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
MS6671_RS29355	59554..59937	-	384	WP_001354030.1	relaxosome protein TraM	-
MS6671_RS29360	60260..60862	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
MS6671_RS29365	61159..61980	-	822	WP_001234445.1	DUF945 domain-containing protein	-
MS6671_RS29370	62091..62387	-	297	WP_001272251.1	hypothetical protein	-
MS6671_RS32895	62687..63064	+	378	Protein_75	hypothetical protein	-
MS6671_RS31545	63302..63460	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	63504..63728	-	225	NuclAT_0	-	Antitoxin
-	63504..63728	-	225	NuclAT_0	-	Antitoxin
-	63504..63728	-	225	NuclAT_0	-	Antitoxin
-	63504..63728	-	225	NuclAT_0	-	Antitoxin
MS6671_RS29380	63740..64459	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
MS6671_RS29385	64456..64890	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MS6671_RS29390	64959..66982	-	2024	Protein_79	ParB/RepB/Spo0J family partition protein	-
MS6671_RS29395	67043..67276	-	234	WP_000006003.1	DUF905 family protein	-
MS6671_RS29400	67334..67861	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / rmtF	-	1..84940	84940

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T285589 WP_001312861.1 NZ_LN824138:c63460-63302 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT285589 NZ_LN824138:c63728-63504 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin