Detailed information of TA system TA285416

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4522707..4523119
• Replicon: chromosome
• Accession: NZ_LM993812
• Organism: Escherichia coli strain K-12 substr. HMS174

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: ECHMS174_RS22330
• Protein ID: WP_000132601.1
• Coordinates: 4522707..4523048 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4523043..4523119 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECHMS174_RS22320	4520120..4521166	-	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
ECHMS174_RS22325	4521166..4522545	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
ECHMS174_RS22330	4522707..4523048	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
-	4523043..4523119	+	77	NuclAT_13	-	Antitoxin
-	4523043..4523119	+	77	NuclAT_13	-	Antitoxin
-	4523043..4523119	+	77	NuclAT_13	-	Antitoxin
-	4523043..4523119	+	77	NuclAT_13	-	Antitoxin
-	4523043..4523119	+	77	NuclAT_14	-	Antitoxin
-	4523043..4523119	+	77	NuclAT_14	-	Antitoxin
-	4523043..4523119	+	77	NuclAT_14	-	Antitoxin
-	4523043..4523119	+	77	NuclAT_14	-	Antitoxin
ECHMS174_RS22335	4523276..4524670	-	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
ECHMS174_RS22340	4524667..4526256	-	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4515622..4524670	9048

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T285416 WP_000132601.1 NZ_LM993812:c4523048-4522707 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

Antitoxin

Nucleotide

• Length: 77 bp

>AT285416 NZ_LM993812:4523043-4523119 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin