Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3921726..3922420 Replicon chromosome
Accession NZ_HG994851
Organism Escherichia coli isolate 127

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag LLO82_RS18910 Protein ID WP_001263500.1
Coordinates 3921726..3922124 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LLO82_RS18915 Protein ID WP_000554758.1
Coordinates 3922127..3922420 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLO82_RS18885 (3917091) 3917091..3917549 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
LLO82_RS18890 (3917810) 3917810..3919267 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
LLO82_RS18895 (3919324) 3919324..3919938 - 615 WP_000602124.1 peptide chain release factor H -
LLO82_RS18900 (3919935) 3919935..3921074 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
LLO82_RS18905 (3921264) 3921264..3921716 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
LLO82_RS18910 (3921726) 3921726..3922124 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LLO82_RS18915 (3922127) 3922127..3922420 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LLO82_RS18920 (3922472) 3922472..3923527 - 1056 WP_001226168.1 DNA polymerase IV -
LLO82_RS18925 (3923598) 3923598..3924383 - 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
LLO82_RS18930 (3924355) 3924355..3926067 + 1713 Protein_3710 flagellar biosynthesis protein FlhA -
LLO82_RS18935 (3926387) 3926387..3926884 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T285329 WP_001263500.1 NZ_HG994851:c3922124-3921726 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT285329 WP_000554758.1 NZ_HG994851:c3922420-3922127 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References