Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3619425..3620104 Replicon chromosome
Accession NZ_HG994851
Organism Escherichia coli isolate 127

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag LLO82_RS17500 Protein ID WP_000057523.1
Coordinates 3619802..3620104 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LLO82_RS17495 Protein ID WP_000806442.1
Coordinates 3619425..3619766 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLO82_RS17485 (3615669) 3615669..3616601 - 933 WP_000883025.1 glutaminase A -
LLO82_RS17490 (3616863) 3616863..3619367 + 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
LLO82_RS17495 (3619425) 3619425..3619766 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LLO82_RS17500 (3619802) 3619802..3620104 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LLO82_RS17505 (3620237) 3620237..3621031 + 795 WP_000365148.1 TraB/GumN family protein -
LLO82_RS17510 (3621235) 3621235..3621714 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LLO82_RS17515 (3621738) 3621738..3622538 + 801 WP_000439798.1 hypothetical protein -
LLO82_RS17520 (3622535) 3622535..3623038 + 504 WP_000667000.1 hypothetical protein -
LLO82_RS17525 (3623076) 3623076..3624728 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T285326 WP_000057523.1 NZ_HG994851:c3620104-3619802 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT285326 WP_000806442.1 NZ_HG994851:c3619766-3619425 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References