Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2554420..2554982 Replicon chromosome
Accession NZ_HG994851
Organism Escherichia coli isolate 127

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag LLO82_RS12170 Protein ID WP_000605675.1
Coordinates 2554420..2554698 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag LLO82_RS12175 Protein ID WP_000781370.1
Coordinates 2554698..2554982 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLO82_RS12145 (2550006) 2550006..2550437 - 432 WP_000152305.1 peroxiredoxin OsmC -
LLO82_RS12150 (2550782) 2550782..2550997 + 216 WP_000495766.1 biofilm-dependent modulation protein -
LLO82_RS12155 (2551099) 2551099..2551236 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
LLO82_RS12160 (2551393) 2551393..2553090 + 1698 WP_000433462.1 malate dehydrogenase -
LLO82_RS12165 (2553224) 2553224..2554234 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
LLO82_RS12170 (2554420) 2554420..2554698 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LLO82_RS12175 (2554698) 2554698..2554982 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
LLO82_RS12180 (2555211) 2555211..2555864 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
LLO82_RS12185 (2555857) 2555857..2556741 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
LLO82_RS12190 (2556754) 2556754..2559801 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T285323 WP_000605675.1 NZ_HG994851:2554420-2554698 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT285323 WP_000781370.1 NZ_HG994851:2554698-2554982 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References