Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3945989..3946683 Replicon chromosome
Accession NZ_HG994850
Organism Escherichia coli isolate 127

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag LLO83_RS19005 Protein ID WP_001263500.1
Coordinates 3945989..3946387 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LLO83_RS19010 Protein ID WP_000554758.1
Coordinates 3946390..3946683 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLO83_RS18980 (3941354) 3941354..3941812 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
LLO83_RS18985 (3942073) 3942073..3943530 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
LLO83_RS18990 (3943587) 3943587..3944201 - 615 WP_000602124.1 peptide chain release factor H -
LLO83_RS18995 (3944198) 3944198..3945337 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
LLO83_RS19000 (3945527) 3945527..3945979 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
LLO83_RS19005 (3945989) 3945989..3946387 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LLO83_RS19010 (3946390) 3946390..3946683 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LLO83_RS19015 (3946735) 3946735..3947790 - 1056 WP_001226168.1 DNA polymerase IV -
LLO83_RS19020 (3947861) 3947861..3948646 - 786 WP_000207578.1 putative lateral flagellar export/assembly protein LafU -
LLO83_RS19025 (3948618) 3948618..3950330 + 1713 Protein_3729 flagellar biosynthesis protein FlhA -
LLO83_RS19030 (3950650) 3950650..3951147 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island blaOXA-48 gmhA/lpcA 3945989..3988215 42226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T285301 WP_001263500.1 NZ_HG994850:c3946387-3945989 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT285301 WP_000554758.1 NZ_HG994850:c3946683-3946390 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References