Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3642913..3643592 Replicon chromosome
Accession NZ_HG994850
Organism Escherichia coli isolate 127

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag LLO83_RS17590 Protein ID WP_000057523.1
Coordinates 3643290..3643592 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LLO83_RS17585 Protein ID WP_000806442.1
Coordinates 3642913..3643254 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLO83_RS17575 (3639157) 3639157..3640089 - 933 WP_000883025.1 glutaminase A -
LLO83_RS17580 (3640351) 3640351..3642855 + 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
LLO83_RS17585 (3642913) 3642913..3643254 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LLO83_RS17590 (3643290) 3643290..3643592 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LLO83_RS17595 (3643725) 3643725..3644519 + 795 WP_000365148.1 TraB/GumN family protein -
LLO83_RS17600 (3644723) 3644723..3645202 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LLO83_RS17605 (3645226) 3645226..3646026 + 801 WP_000439798.1 hypothetical protein -
LLO83_RS17610 (3646023) 3646023..3646526 + 504 WP_000667000.1 hypothetical protein -
LLO83_RS17615 (3646564) 3646564..3648216 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T285298 WP_000057523.1 NZ_HG994850:c3643592-3643290 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT285298 WP_000806442.1 NZ_HG994850:c3643254-3642913 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References