Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2577908..2578470 Replicon chromosome
Accession NZ_HG994850
Organism Escherichia coli isolate 127

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag LLO83_RS12260 Protein ID WP_000605675.1
Coordinates 2577908..2578186 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag LLO83_RS12265 Protein ID WP_000781370.1
Coordinates 2578186..2578470 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLO83_RS12235 (2573494) 2573494..2573925 - 432 WP_000152305.1 peroxiredoxin OsmC -
LLO83_RS12240 (2574270) 2574270..2574485 + 216 WP_000495766.1 biofilm-dependent modulation protein -
LLO83_RS12245 (2574587) 2574587..2574724 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
LLO83_RS12250 (2574881) 2574881..2576578 + 1698 WP_000433462.1 malate dehydrogenase -
LLO83_RS12255 (2576712) 2576712..2577722 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
LLO83_RS12260 (2577908) 2577908..2578186 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LLO83_RS12265 (2578186) 2578186..2578470 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
LLO83_RS12270 (2578699) 2578699..2579352 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
LLO83_RS12275 (2579345) 2579345..2580229 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
LLO83_RS12280 (2580242) 2580242..2583289 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T285295 WP_000605675.1 NZ_HG994850:2577908-2578186 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT285295 WP_000781370.1 NZ_HG994850:2578186-2578470 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References