Detailed information of TA system TA285214

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22690..23116
• Replicon: plasmid pEC958
• Accession: NZ_HG941719
• Organism: Escherichia coli O25b:H4-ST131 strain EC958

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EC958_RS25735
• Protein ID: WP_001312861.1
• Coordinates: 22958..23116 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22690..22914 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC958_RS25705	18682..19167	+	486	WP_042029316.1	single-stranded DNA-binding protein	-
EC958_RS25710	19223..19456	+	234	WP_000006004.1	DUF905 domain-containing protein	-
EC958_RS25715	19515..21473	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
EC958_RS25720	21528..21962	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
EC958_RS25725	21959..22678	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
EC958_RS29470	22690..22878	-	189	WP_001299721.1	hypothetical protein	-
-	22690..22914	+	225	NuclAT_0	-	Antitoxin
-	22690..22914	+	225	NuclAT_0	-	Antitoxin
-	22690..22914	+	225	NuclAT_0	-	Antitoxin
-	22690..22914	+	225	NuclAT_0	-	Antitoxin
EC958_RS25735	22958..23116	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
EC958_RS25745	24035..24322	+	288	WP_000107537.1	hypothetical protein	-
EC958_RS25750	24443..25264	+	822	WP_001234469.1	DUF945 domain-containing protein	-
EC958_RS25755	25563..26210	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
EC958_RS25760	26496..26879	+	384	WP_001151564.1	relaxosome protein TraM	-
EC958_RS25765	27073..27759	+	687	WP_000332487.1	PAS domain-containing protein	-
EC958_RS25770	27853..28080	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A)	-	1..135602	135602

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T285214 WP_001312861.1 NZ_HG941719:22958-23116 [Escherichia coli O25b:H4-ST131]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT285214 NZ_HG941719:22690-22914 [Escherichia coli O25b:H4-ST131]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin