Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 538457..539136 Replicon chromosome
Accession NZ_HG941718
Organism Escherichia coli O25b:H4-ST131 strain EC958

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag EC958_RS02530 Protein ID WP_000057523.1
Coordinates 538457..538759 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag EC958_RS02535 Protein ID WP_000806442.1
Coordinates 538795..539136 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC958_RS02505 533833..535485 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
EC958_RS02510 535523..536026 - 504 WP_000667000.1 hypothetical protein -
EC958_RS02515 536023..536823 - 801 WP_000439798.1 hypothetical protein -
EC958_RS02520 536847..537326 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
EC958_RS02525 537530..538324 - 795 WP_000365147.1 TraB/GumN family protein -
EC958_RS02530 538457..538759 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EC958_RS02535 538795..539136 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
EC958_RS02540 539194..541698 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
EC958_RS02545 541960..542892 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T285188 WP_000057523.1 NZ_HG941718:538457-538759 [Escherichia coli O25b:H4-ST131]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT285188 WP_000806442.1 NZ_HG941718:538795-539136 [Escherichia coli O25b:H4-ST131]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References