Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2188024..2188892 Replicon chromosome
Accession NZ_HG813240
Organism Mycobacterium tuberculosis 49-02

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag MT49_RS10170 Protein ID WP_010886136.1
Coordinates 2188024..2188401 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag MT49_RS10175 Protein ID WP_003409886.1
Coordinates 2188443..2188892 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT49_RS10120 2183813..2184265 - 453 WP_003899095.1 lipoprotein -
MT49_RS10125 2184329..2184730 + 402 WP_003409869.1 hypothetical protein -
MT49_RS10130 2184723..2184905 - 183 WP_003409870.1 hypothetical protein -
MT49_RS10135 2185019..2185369 - 351 WP_003409871.1 hypothetical protein -
MT49_RS10140 2185380..2186282 - 903 WP_003899097.1 hypothetical protein -
MT49_RS10145 2186303..2186494 - 192 WP_003409876.1 hypothetical protein -
MT49_RS10150 2186495..2186791 - 297 WP_003409877.1 hypothetical protein -
MT49_RS10155 2187031..2187246 + 216 WP_003409878.1 antitoxin -
MT49_RS10160 2187243..2187554 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
MT49_RS22495 2187528..2188049 - 522 WP_003904745.1 hypothetical protein -
MT49_RS10170 2188024..2188401 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
MT49_RS10175 2188443..2188892 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MT49_RS10180 2188889..2189434 + 546 WP_003409891.1 SecB-like chaperone -
MT49_RS10185 2189323..2189937 - 615 WP_003901296.1 hypothetical protein -
MT49_RS10190 2189986..2190282 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
MT49_RS10195 2190279..2190530 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
MT49_RS10200 2190517..2191011 + 495 WP_003899099.1 hypothetical protein -
MT49_RS10205 2191171..2191578 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
MT49_RS10210 2191582..2191854 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MT49_RS10215 2191887..2193107 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T285084 WP_010886136.1 NZ_HG813240:2188024-2188401 [Mycobacterium tuberculosis 49-02]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT285084 WP_003409886.1 NZ_HG813240:2188443-2188892 [Mycobacterium tuberculosis 49-02]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References