Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4997181..4997767 Replicon chromosome
Accession NZ_CP128550
Organism Pseudomonas alloputida strain NMI5594_09

Toxin (Protein)


Gene name graT Uniprot ID Q88MI5
Locus tag LU693_RS23155 Protein ID WP_010952670.1
Coordinates 4997181..4997459 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88MI6
Locus tag LU693_RS23160 Protein ID WP_010952669.1
Coordinates 4997468..4997767 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU693_RS23145 (LU693_023145) 4993982..4995178 + 1197 WP_010952672.1 succinyldiaminopimelate transaminase -
LU693_RS23150 (LU693_023150) 4995284..4996930 - 1647 WP_049587427.1 Na+/H+ antiporter -
LU693_RS23155 (LU693_023155) 4997181..4997459 + 279 WP_010952670.1 type II toxin-antitoxin system toxin GraT Toxin
LU693_RS23160 (LU693_023160) 4997468..4997767 + 300 WP_010952669.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
LU693_RS23165 (LU693_023165) 4997892..4998239 + 348 WP_003252272.1 ArsC family reductase -
LU693_RS23170 (LU693_023170) 4998271..4999305 + 1035 WP_004574732.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
LU693_RS23175 (LU693_023175) 4999387..5000592 + 1206 WP_010952615.1 cysteine desulfurase -
LU693_RS23180 (LU693_023180) 5000589..5000999 + 411 WP_003252266.1 SufE family protein -
LU693_RS23185 (LU693_023185) 5001112..5001921 - 810 WP_162490563.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10446.72 Da        Isoelectric Point: 7.2450

>T284875 WP_010952670.1 NZ_CP128550:4997181-4997459 [Pseudomonas alloputida]
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10905.55 Da        Isoelectric Point: 5.6793

>AT284875 WP_010952669.1 NZ_CP128550:4997468-4997767 [Pseudomonas alloputida]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6F8S

Antitoxin

Source ID Structure
PDB 6F8S
AlphaFold DB Q88MI6

References