Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1449666..1450267 Replicon chromosome
Accession NZ_CP128550
Organism Pseudomonas alloputida strain NMI5594_09

Toxin (Protein)


Gene name higB Uniprot ID A0A2L1IAQ2
Locus tag LU693_RS06610 Protein ID WP_049587984.1
Coordinates 1449953..1450267 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2L1IAR7
Locus tag LU693_RS06605 Protein ID WP_049587986.1
Coordinates 1449666..1449953 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU693_RS06585 (LU693_006585) 1444698..1445543 + 846 WP_003254799.1 DUF6502 family protein -
LU693_RS06590 (LU693_006590) 1445540..1447258 + 1719 WP_010952348.1 DUF5666 domain-containing protein -
LU693_RS06595 (LU693_006595) 1447313..1448062 + 750 WP_049587988.1 hypothetical protein -
LU693_RS06600 (LU693_006600) 1448134..1449465 - 1332 WP_004576169.1 30S ribosomal protein S12 methylthiotransferase RimO -
LU693_RS06605 (LU693_006605) 1449666..1449953 - 288 WP_049587986.1 helix-turn-helix domain-containing protein Antitoxin
LU693_RS06610 (LU693_006610) 1449953..1450267 - 315 WP_049587984.1 hypothetical protein Toxin
LU693_RS06615 (LU693_006615) 1450716..1452626 + 1911 WP_010952353.1 potassium transporter Kup -
LU693_RS06620 (LU693_006620) 1452675..1453967 - 1293 WP_010952354.1 AcvB/VirJ family lysyl-phosphatidylglycerol hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-88)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11784.61 Da        Isoelectric Point: 9.9097

>T284871 WP_049587984.1 NZ_CP128550:c1450267-1449953 [Pseudomonas alloputida]
MIFIETPVFTSDLKEHLDDEEYRALQAYLAEHPEAGSLLEETGGLRKIRWAAKGKGKSGGVRVIYYHVTAAHQIRMILIY
RKGIVDTLTSSQKAQLRALNKGWK

Download         Length: 315 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10841.31 Da        Isoelectric Point: 7.4063

>AT284871 WP_049587986.1 NZ_CP128550:c1449953-1449666 [Pseudomonas alloputida]
MEKHLFDRLVESMTQMDEIDRGERQPSREFHVDALQVKKIRQATGLSQAAFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALS

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1IAQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1IAR7

References