Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5391317..5392107 Replicon chromosome
Accession NZ_CP128540
Organism Pseudomonas alloputida strain NMI24_14

Toxin (Protein)


Gene name yhaV Uniprot ID Q88NE5
Locus tag LU682_RS25050 Protein ID WP_010952397.1
Coordinates 5391640..5392107 (+) Length 156 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID I7C311
Locus tag LU682_RS25045 Protein ID WP_014859917.1
Coordinates 5391317..5391643 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU682_RS25025 (LU682_025025) 5386851..5387912 - 1062 WP_049587435.1 HlyD family secretion protein -
LU682_RS25030 (LU682_025030) 5387941..5389482 - 1542 WP_010952401.1 MFS transporter -
LU682_RS25035 (LU682_025035) 5389599..5390504 - 906 WP_010952400.1 LysR family transcriptional regulator -
LU682_RS25040 (LU682_025040) 5390778..5391224 + 447 WP_010952399.1 universal stress protein -
LU682_RS25045 (LU682_025045) 5391317..5391643 + 327 WP_014859917.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
LU682_RS25050 (LU682_025050) 5391640..5392107 + 468 WP_010952397.1 type II toxin-antitoxin system YhaV family toxin Toxin
LU682_RS25055 (LU682_025055) 5392180..5393040 - 861 WP_010952396.1 HlyD family secretion protein -
LU682_RS25060 (LU682_025060) 5393051..5393251 - 201 WP_003251718.1 DUF1656 domain-containing protein -
LU682_RS25065 (LU682_025065) 5393241..5395418 - 2178 WP_003251716.1 FUSC family protein -
LU682_RS25070 (LU682_025070) 5395415..5396944 - 1530 WP_010952395.1 efflux transporter outer membrane subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(17-152)

Antitoxin

PrlF antitoxin

(8-106)


Sequences

Toxin        


Download         Length: 156 a.a.        Molecular weight: 17962.34 Da        Isoelectric Point: 9.7963

>T284837 WP_010952397.1 NZ_CP128540:5391640-5392107 [Pseudomonas alloputida]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWINDESSKRAYESSDDAYKVFQKMLHSGNPPDDWDQLLQEAAAD

Download         Length: 468 bp

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11919.40 Da        Isoelectric Point: 4.3722

>AT284837 WP_014859917.1 NZ_CP128540:5391317-5391643 [Pseudomonas alloputida]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Download         Length: 327 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88NE5

Antitoxin

Source ID Structure
AlphaFold DB I7C311

References