284836

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5008407..5008993 Replicon chromosome
Accession NZ_CP128540
Organism Pseudomonas alloputida strain NMI24_14

Toxin (Protein)


Gene name graT Uniprot ID Q88MI5
Locus tag LU682_RS23225 Protein ID WP_010952670.1
Coordinates 5008407..5008685 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88MI6
Locus tag LU682_RS23230 Protein ID WP_010952669.1
Coordinates 5008694..5008993 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LU682_RS23215 (LU682_023215) 5005208..5006404 + 1197 WP_010952672.1 succinyldiaminopimelate transaminase -
LU682_RS23220 (LU682_023220) 5006510..5008156 - 1647 WP_049587427.1 Na+/H+ antiporter -
LU682_RS23225 (LU682_023225) 5008407..5008685 + 279 WP_010952670.1 type II toxin-antitoxin system toxin GraT Toxin
LU682_RS23230 (LU682_023230) 5008694..5008993 + 300 WP_010952669.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
LU682_RS23235 (LU682_023235) 5009118..5009465 + 348 WP_003252272.1 ArsC family reductase -
LU682_RS23240 (LU682_023240) 5009497..5010531 + 1035 WP_004574732.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
LU682_RS23245 (LU682_023245) 5010613..5011818 + 1206 WP_010952615.1 cysteine desulfurase -
LU682_RS23250 (LU682_023250) 5011815..5012225 + 411 WP_003252266.1 SufE family protein -
LU682_RS23255 (LU682_023255) 5012338..5013147 - 810 WP_162490563.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10446.72 Da        Isoelectric Point: 7.2450

>T284836 WP_010952670.1 NZ_CP128540:5008407-5008685 [Pseudomonas alloputida]
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10905.55 Da        Isoelectric Point: 5.6793

>AT284836 WP_010952669.1 NZ_CP128540:5008694-5008993 [Pseudomonas alloputida]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6F8S

Antitoxin

Source ID Structure
AlphaFold DB Q88MI6

References