Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 6023440..6024032 Replicon chromosome
Accession NC_019670
Organism Pseudomonas sp. UW4

Toxin (Protein)


Gene name graT Uniprot ID J3FH26
Locus tag PPUTUW4_RS26865 Protein ID WP_007980828.1
Coordinates 6023754..6024032 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID J2PV29
Locus tag PPUTUW4_RS26860 Protein ID WP_007980826.1
Coordinates 6023440..6023745 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPUTUW4_RS26830 6019029..6019352 - 324 WP_015097343.1 hypothetical protein -
PPUTUW4_RS26835 6019454..6019879 - 426 WP_015097344.1 secondary thiamine-phosphate synthase enzyme YjbQ -
PPUTUW4_RS26840 6020094..6021431 - 1338 WP_015097345.1 ammonium transporter -
PPUTUW4_RS26845 6021467..6021805 - 339 WP_002555808.1 P-II family nitrogen regulator -
PPUTUW4_RS26850 6022225..6022485 + 261 WP_007980822.1 accessory factor UbiK family protein -
PPUTUW4_RS26855 6022868..6023389 + 522 WP_015097346.1 Bro-N domain-containing protein -
PPUTUW4_RS26860 6023440..6023745 - 306 WP_007980826.1 HigA family addiction module antidote protein Antitoxin
PPUTUW4_RS26865 6023754..6024032 - 279 WP_007980828.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PPUTUW4_RS26870 6024304..6025797 + 1494 WP_015097347.1 YifB family Mg chelatase-like AAA ATPase -
PPUTUW4_RS26875 6025880..6026800 - 921 WP_015097348.1 LysR family transcriptional regulator -
PPUTUW4_RS26880 6026959..6028356 + 1398 WP_015097349.1 NorM family multidrug efflux MATE transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-83)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10585.25 Da        Isoelectric Point: 8.5043

>T28482 WP_007980828.1 NC_019670:c6024032-6023754 [Pseudomonas sp. UW4]
MIVSFKCSETEYLFRNGKTRMWSAILSVVERKLTMLDAAAVLIDLRSPPGNRLEALEGNRKGQYSIRINAQWRICFIWGP
NGPENVEIVDYH

Download         Length: 279 bp

>T28482 NC_019670:c6024032-6023754 [Pseudomonas sp. UW4]
ATGATCGTTAGCTTTAAGTGTTCTGAGACCGAGTACCTGTTTCGCAACGGTAAGACCCGTATGTGGTCAGCCATTCTGAG
CGTCGTGGAACGAAAGCTGACGATGCTTGATGCTGCTGCCGTGTTGATAGACCTTCGATCCCCGCCGGGTAATCGCCTGG
AGGCGCTGGAGGGGAATCGAAAAGGTCAGTACAGCATCCGTATCAACGCTCAATGGCGTATTTGCTTCATTTGGGGTCCT
AACGGACCAGAGAATGTCGAAATCGTCGATTATCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11245.97 Da        Isoelectric Point: 6.4556

>AT28482 WP_007980826.1 NC_019670:c6023745-6023440 [Pseudomonas sp. UW4]
MTKNGMRPVHPGEILKEEYLEPLSLTAAALARALNVSTPTVNDIVLQRRGVSADMALRLSICLDTTPEFWLNLQSTYDLR
KAEIERGAAIRDQVERLAHCA

Download         Length: 306 bp

>AT28482 NC_019670:c6023745-6023440 [Pseudomonas sp. UW4]
ATGACTAAGAATGGTATGCGCCCGGTTCACCCTGGCGAGATCCTCAAAGAGGAATACCTTGAGCCTCTGAGCCTTACGGC
TGCCGCTCTGGCAAGAGCATTGAATGTGTCGACACCTACGGTGAACGACATCGTGCTCCAGCGTCGGGGTGTCAGTGCTG
ATATGGCTCTAAGGTTATCTATTTGTCTGGACACTACCCCGGAATTTTGGCTGAACCTGCAATCAACTTACGATTTGCGT
AAAGCCGAGATTGAACGAGGGGCTGCGATTCGGGATCAGGTTGAGCGGCTTGCACACTGTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J3FH26

Antitoxin

Source ID Structure
AlphaFold DB J2PV29

References