Detailed information of TA system TA028481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5657298..5657961
• Replicon: chromosome
• Accession: NC_019670
• Organism: Pseudomonas sp. UW4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K9NS96
• Locus tag: PPUTUW4_RS25175
• Protein ID: WP_015097075.1
• Coordinates: 5657596..5657961 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: J2QEB2
• Locus tag: PPUTUW4_RS25170
• Protein ID: WP_007980287.1
• Coordinates: 5657298..5657603 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPUTUW4_RS25160	5653385..5657131	+	3747	WP_015097074.1	bifunctional diguanylate cyclase/phosphodiesterase	-
PPUTUW4_RS25170	5657298..5657603	-	306	WP_007980287.1	XRE family transcriptional regulator	Antitoxin
PPUTUW4_RS25175	5657596..5657961	-	366	WP_015097075.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPUTUW4_RS25180	5658540..5658860	+	321	WP_015097076.1	DUF3077 domain-containing protein	-
PPUTUW4_RS25185	5658929..5659531	+	603	WP_015097077.1	nucleotidyltransferase domain-containing protein	-
PPUTUW4_RS25190	5659521..5659949	+	429	WP_015097078.1	hypothetical protein	-
PPUTUW4_RS25195	5659965..5660645	-	681	WP_041475085.1	peptidase M41	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (6-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14099.64 Da
• Isoelectric Point: 4.8330

>T28481 WP_015097075.1 NC_019670:c5657961-5657596 [Pseudomonas sp. UW4]
MKWDVEYTDEFEAWWDRLNEAEQDSVQATVMLLGEDGPHLGFPHTSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRSA
ILLIGGDKTGQERWYAEHVPLAERLYDEHLETLMKEGYDNG

Nucleotide

• Length: 366 bp

>T28481 NC_019670:c5657961-5657596 [Pseudomonas sp. UW4]
ATGAAATGGGACGTCGAATACACGGATGAATTTGAGGCCTGGTGGGATCGCCTGAACGAGGCCGAACAGGACTCGGTTCA
GGCCACGGTGATGTTGCTGGGCGAGGATGGCCCACACTTGGGTTTTCCACATACCAGCGATATCAAGGGTTCACGGCATG
GCAACCTCCGTGAGTTAAGGGTACAGCATGCTGGCAGGCCGTACCGTGTGCTGTACGCGTTTGATCCACGGCGCAGCGCG
ATCCTGCTGATTGGCGGTGATAAAACCGGCCAGGAGCGTTGGTATGCGGAACACGTGCCACTGGCCGAGCGTCTCTACGA
CGAGCACTTGGAAACATTGATGAAAGAGGGTTATGACAATGGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11494.26 Da
• Isoelectric Point: 10.4463

>AT28481 WP_007980287.1 NC_019670:c5657603-5657298 [Pseudomonas sp. UW4]
MAKKFADLVARRSPESQARVEALHQKLRAQMPLHELRQAQALSQDTLARTLHVNQAAISKMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVKIENFAS

Nucleotide

• Length: 306 bp

>AT28481 NC_019670:c5657603-5657298 [Pseudomonas sp. UW4]
ATGGCTAAAAAATTTGCTGATTTGGTGGCACGTCGCTCTCCAGAGTCTCAAGCGCGTGTCGAGGCCTTGCATCAGAAGCT
ACGAGCTCAAATGCCGTTGCACGAGTTGCGTCAGGCGCAAGCGTTGAGTCAGGACACACTGGCCAGGACGCTGCATGTCA
ACCAGGCGGCGATCTCCAAGATGGAGCGGCGAACTGACATGTACATCAGTACGTTGCGCAACTACATCCGCGCCATGGGG
GGCGAGTTGGAAATCATCGCCACTTTCCCCGAGGGCCAGGTAAAGATCGAGAACTTTGCCAGCTGA

Structures

Toxin

• AlphaFold DB: K9NS96

Antitoxin

• AlphaFold DB: J2QEB2