Detailed information of TA system TA028464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 85227..85885
• Replicon: chromosome
• Accession: NC_019395
• Organism: Propionibacterium acidipropionici ATCC 4875

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: K7RJ67
• Locus tag: PACID_RS00365
• Protein ID: WP_015068846.1
• Coordinates: 85535..85885 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: PACID_RS00360
• Protein ID: WP_198027853.1
• Coordinates: 85227..85538 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PACID_RS00340	80380..81804	-	1425	WP_160165414.1	PKD domain-containing protein	-
PACID_RS00345	81932..83188	+	1257	WP_051143434.1	IS256 family transposase	-
PACID_RS00350	83191..84057	-	867	WP_051143435.1	hypothetical protein	-
PACID_RS00355	84408..85061	-	654	WP_015068844.1	hypothetical protein	-
PACID_RS00360	85227..85538	-	312	WP_198027853.1	helix-turn-helix domain-containing protein	Antitoxin
PACID_RS00365	85535..85885	-	351	WP_015068846.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PACID_RS00370	86066..86263	+	198	WP_015068847.1	hypothetical protein	-
PACID_RS00375	86372..87079	-	708	WP_015068848.1	(2Fe-2S) ferredoxin domain-containing protein	-
PACID_RS00380	87066..87875	-	810	WP_015068849.1	ABC transporter ATP-binding protein	-
PACID_RS00385	87872..88936	-	1065	WP_015068850.1	iron chelate uptake ABC transporter family permease subunit	-
PACID_RS00390	88933..90015	-	1083	WP_148279593.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (11-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-90)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13220.22 Da
• Isoelectric Point: 10.2579

>T28464 WP_015068846.1 NC_019395:c85885-85535 [Acidipropionibacterium acidipropionici ATCC 4875]
MWSADLTLIADWLAGLDEGSQVQVVAAIELLKSIGPQLGRPLVDTVKASRHKNMKELRPGSSGRTELRILFAFDPRRKAI
LLIAGDKSNDWDRWYRRNIPLADDLFDEHLRALRRS

Nucleotide

• Length: 351 bp

>T28464 NC_019395:c85885-85535 [Acidipropionibacterium acidipropionici ATCC 4875]
ATGTGGAGCGCCGACCTCACACTCATCGCCGACTGGCTTGCCGGGCTGGACGAGGGATCACAGGTTCAGGTCGTCGCCGC
CATAGAACTGTTGAAGAGCATCGGTCCTCAACTCGGTCGCCCTCTCGTCGACACTGTCAAAGCATCACGGCACAAGAACA
TGAAGGAACTCCGACCAGGCTCATCAGGCCGAACCGAGCTGCGCATCCTGTTCGCCTTCGATCCGAGACGCAAGGCGATC
CTGCTCATCGCCGGCGACAAGAGCAATGACTGGGATCGGTGGTACCGACGCAACATCCCCCTCGCCGACGACCTGTTCGA
CGAACACCTACGAGCACTGAGAAGGTCATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11484.21 Da
• Isoelectric Point: 5.7484

>AT28464 WP_198027853.1 NC_019395:c85538-85227 [Acidipropionibacterium acidipropionici ATCC 4875]
MIMGLSLEEFLAEHPVDRDQVEAAKAEMLARVRAYRLRELREAAHLTQAQVAEKIGVSQRQVSKIETGDLNNSKLITVRK
YMEAVGGDLAVEFVSGDMRVQVA

Nucleotide

• Length: 312 bp

>AT28464 NC_019395:c85538-85227 [Acidipropionibacterium acidipropionici ATCC 4875]
ATGATCATGGGACTGTCCTTGGAAGAGTTTCTCGCTGAACACCCCGTGGACCGCGACCAGGTCGAGGCTGCCAAGGCCGA
GATGCTGGCTCGGGTCCGCGCCTACCGGCTCCGTGAACTGCGCGAGGCCGCCCACCTCACTCAGGCCCAGGTGGCCGAGA
AGATCGGAGTCTCCCAGCGCCAGGTTTCCAAGATCGAGACCGGCGACCTCAACAACTCCAAGCTCATCACCGTACGCAAG
TACATGGAGGCTGTCGGTGGCGACCTGGCCGTCGAGTTCGTCTCCGGTGACATGCGCGTCCAAGTGGCGTGA

Structures

Toxin

• AlphaFold DB: K7RJ67