Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 69048..69775 Replicon plasmid pYZEF2-2C_tmex
Accession NZ_CP128484
Organism Klebsiella pneumoniae strain YZEF2-2C

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag QTN20_RS00350 Protein ID WP_011251285.1
Coordinates 69464..69775 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QTN20_RS00345 Protein ID WP_011251286.1
Coordinates 69048..69467 (-) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTN20_RS00325 (QTN20_00325) 64573..65334 - 762 WP_017900871.1 histidine utilization repressor -
QTN20_RS00330 (QTN20_00330) 65437..66804 + 1368 WP_032437385.1 formimidoylglutamate deiminase -
QTN20_RS00335 (QTN20_00335) 67329..67864 + 536 Protein_66 transposase -
QTN20_RS00340 (QTN20_00340) 67933..68901 - 969 WP_077268729.1 IS5 family transposase -
QTN20_RS00345 (QTN20_00345) 69048..69467 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
QTN20_RS00350 (QTN20_00350) 69464..69775 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
QTN20_RS00355 (QTN20_00355) 69980..70417 - 438 Protein_70 DDE-type integrase/transposase/recombinase -
QTN20_RS00360 (QTN20_00360) 70531..70869 + 339 WP_023288266.1 transposase -
QTN20_RS00365 (QTN20_00365) 70866..71213 + 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
QTN20_RS00370 (QTN20_00370) 71262..72790 + 1529 Protein_73 IS66 family transposase -
QTN20_RS00375 (QTN20_00375) 72830..73009 + 180 Protein_74 IS5/IS1182 family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 67470..77699 10229
- inside Non-Mobilizable plasmid aph(3'')-Ib / aph(6)-Id / aph(4)-Ia / aac(3)-IVa / sul3 / ant(3'')-Ia / cmlA1 / aadA2 - 1..207962 207962


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T284630 WP_011251285.1 NZ_CP128484:c69775-69464 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp


Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT284630 WP_011251286.1 NZ_CP128484:c69467-69048 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References