Detailed information of TA system TA028451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 575702..576315
• Replicon: chromosome
• Accession: NC_019382
• Organism: Bordetella bronchiseptica 253

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: BN112_RS02845
• Protein ID: WP_003811545.1
• Coordinates: 576004..576315 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BN112_RS02840
• Protein ID: WP_003811547.1
• Coordinates: 575702..576001 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN112_RS02830	571810..572484	-	675	WP_015063801.1	response regulator transcription factor	-
BN112_RS02835	572614..575688	+	3075	WP_015063802.1	autotransporter SphB2	-
BN112_RS02840	575702..576001	-	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
BN112_RS02845	576004..576315	-	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BN112_RS02850	576429..577052	-	624	WP_003811542.1	glutathione S-transferase family protein	-
BN112_RS02855	577171..578124	-	954	WP_015063803.1	LysR family transcriptional regulator	-
BN112_RS02860	578240..579202	+	963	WP_003811538.1	tripartite tricarboxylate transporter substrate binding protein	-
BN112_RS02865	579217..580611	+	1395	WP_010926625.1	arylsulfatase	-
BN112_RS02870	580608..581174	+	567	WP_015039900.1	LamB/YcsF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T28451 WP_003811545.1 NC_019382:c576315-576004 [Bordetella bronchiseptica 253]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T28451 NC_019382:c576315-576004 [Bordetella bronchiseptica 253]
ATGGAGTTCATCGAAACCCCAATTTTCACGCAATTGATCACCGCGTTGCTGTCGGATGAGGAATATTCCAGGCTGCAACA
GCTGCTGATCGACAACCCCGAAAGGGGCGATCTGCTGCGTGGGGGCGGCGGTATACGAAAGATGCGTTACGGCCGGCAAG
GCACGGGCAAGCGAGGTGGCATCCGGGTCATTTATTACTGGATCAGCCAGGATTGTCAGATCTACATGCTTGCGGCTTAT
GCAAAGTCGAAGAAGATCAACCTGACCCCGGACGAAATCGCGGCGCTGCGCGAACTGGTCAAGGAGCTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT28451 WP_003811547.1 NC_019382:c576001-575702 [Bordetella bronchiseptica 253]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT28451 NC_019382:c576001-575702 [Bordetella bronchiseptica 253]
ATGAAAAAAGACAACACGGTGTTCAACGACCTGCAAGCCAGCCTGAAGCAAGCCGTGGCCATTCGCCAAGGCAAGATGCC
GCCATCGCGTGTATTCAAGGTTGAGACTGTGGATGTGAAGGCAACGCGCGAAAAAACGCACATGTCTCAAACGGAATTCG
CCAAAGCCATCCACGTCAGTGTCCGTACGCTGCAGAACTGGGAGCAAGGTCACCGCAATCCGACGGGCCCTGCCGTGGCG
CTTCTGAAAATCGTGGCAAGCGCCCCGGACATTGCCGTGCGAGCGCTGCGGACCGCGTAA

Structures

Toxin

• AlphaFold DB: K0MEM3