Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 105568..106109 Replicon plasmid F
Accession NZ_CP128219
Organism Escherichia coli strain TG1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag QSI84_RS22340 Protein ID WP_000615983.1
Coordinates 105831..106109 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag QSI84_RS22335 Protein ID WP_000729703.1
Coordinates 105568..105828 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QSI84_RS22315 (101243) 101243..102028 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
QSI84_RS22320 (102000) 102000..103712 + 1713 Protein_107 flagellar biosynthesis protein FlhA -
QSI84_RS22325 (103936) 103936..104433 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
QSI84_RS22330 (104609) 104609..105358 - 750 WP_000056849.1 C40 family peptidase -
QSI84_RS22335 (105568) 105568..105828 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
QSI84_RS22340 (105831) 105831..106109 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
QSI84_RS22345 (106265) 106265..107005 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
QSI84_RS22350 (106976) 106976..107743 - 768 WP_000333380.1 class II glutamine amidotransferase -
QSI84_RS22355 (108018) 108018..108557 - 540 WP_001096705.1 DUF2058 domain-containing protein -
QSI84_RS22360 (108659) 108659..109870 - 1212 WP_000107627.1 3-(3-hydroxy-phenyl)propionate transporter -
QSI84_RS22365 (110034) 110034..110203 - 170 Protein_116 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE 1..232324 232324


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T284437 WP_000615983.1 NZ_CP128219:105831-106109 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT284437 WP_000729703.1 NZ_CP128219:105568-105828 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References