Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1091297..1092024 Replicon chromosome
Accession NZ_CP127848
Organism Escherichia coli strain MB7671

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag PJZ59_RS05340 Protein ID WP_000547564.1
Coordinates 1091297..1091608 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PJZ59_RS05345 Protein ID WP_000126294.1
Coordinates 1091605..1092024 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PJZ59_RS05310 (1086439) 1086439..1088148 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
PJZ59_RS05315 (1088158) 1088158..1088700 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
PJZ59_RS05320 (1088700) 1088700..1089467 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
PJZ59_RS05325 (1089464) 1089464..1089874 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
PJZ59_RS05330 (1089867) 1089867..1090337 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
PJZ59_RS05335 (1090362) 1090362..1091123 + 762 WP_001026446.1 hypothetical protein -
PJZ59_RS05340 (1091297) 1091297..1091608 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
PJZ59_RS05345 (1091605) 1091605..1092024 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
PJZ59_RS05350 (1092138) 1092138..1093562 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
PJZ59_RS05355 (1093571) 1093571..1095028 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
PJZ59_RS05360 (1095288) 1095288..1096298 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
PJZ59_RS05365 (1096447) 1096447..1096974 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T284281 WP_000547564.1 NZ_CP127848:1091297-1091608 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT284281 WP_000126294.1 NZ_CP127848:1091605-1092024 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References