Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 653923..654485 Replicon chromosome
Accession NZ_CP127316
Organism Escherichia coli strain Ch1

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag QRM71_RS03305 Protein ID WP_000605676.1
Coordinates 654207..654485 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QRM71_RS03300 Protein ID WP_000781370.1
Coordinates 653923..654207 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM71_RS03285 (649282) 649282..652329 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
QRM71_RS03290 (652342) 652342..653226 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
QRM71_RS03295 (653219) 653219..653872 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
QRM71_RS03300 (653923) 653923..654207 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QRM71_RS03305 (654207) 654207..654485 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QRM71_RS03310 (654671) 654671..655681 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
QRM71_RS03315 (655815) 655815..657512 - 1698 WP_000433462.1 malate dehydrogenase -
QRM71_RS03320 (657669) 657669..657806 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QRM71_RS03325 (657908) 657908..658123 - 216 WP_001595746.1 biofilm-dependent modulation protein -
QRM71_RS03330 (658468) 658468..658899 + 432 WP_000152303.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 659091..659549 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T284168 WP_000605676.1 NZ_CP127316:c654485-654207 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT284168 WP_000781370.1 NZ_CP127316:c654207-653923 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References