Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1571949..1572676 Replicon chromosome
Accession NZ_CP127314
Organism Escherichia coli strain C25

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag QRM69_RS07545 Protein ID WP_000547555.1
Coordinates 1571949..1572260 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag QRM69_RS07550 Protein ID WP_000126293.1
Coordinates 1572257..1572676 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRM69_RS07515 (1567090) 1567090..1568799 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
QRM69_RS07520 (1568809) 1568809..1569351 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
QRM69_RS07525 (1569351) 1569351..1570118 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
QRM69_RS07530 (1570115) 1570115..1570525 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
QRM69_RS07535 (1570518) 1570518..1570988 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
QRM69_RS07540 (1571031) 1571031..1571786 + 756 WP_001305268.1 hypothetical protein -
QRM69_RS07545 (1571949) 1571949..1572260 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
QRM69_RS07550 (1572257) 1572257..1572676 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
QRM69_RS07555 (1572768) 1572768..1573196 - 429 WP_286005147.1 hypothetical protein -
QRM69_RS07560 (1573581) 1573581..1574108 + 528 WP_001078777.1 electron transport protein HydN -
QRM69_RS07565 (1574261) 1574261..1576513 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T284140 WP_000547555.1 NZ_CP127314:1571949-1572260 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT284140 WP_000126293.1 NZ_CP127314:1572257-1572676 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References