Detailed information of TA system TA028414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2729580..2730193
• Replicon: chromosome
• Accession: NC_018828
• Organism: Bordetella parapertussis Bpp5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: BN117_RS13040
• Protein ID: WP_003811545.1
• Coordinates: 2729882..2730193 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BN117_RS13035
• Protein ID: WP_003811547.1
• Coordinates: 2729580..2729879 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN117_RS13025	2726219..2726893	-	675	WP_015039897.1	response regulator transcription factor	-
BN117_RS13030	2727023..2729566	+	2544	Protein_2561	autotransporter domain-containing protein	-
BN117_RS13035	2729580..2729879	-	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
BN117_RS13040	2729882..2730193	-	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BN117_RS13045	2730307..2730930	-	624	WP_015039898.1	glutathione S-transferase family protein	-
BN117_RS13050	2731049..2732000	-	952	Protein_2565	LysR family transcriptional regulator	-
BN117_RS13055	2732116..2733078	+	963	WP_015039899.1	tripartite tricarboxylate transporter substrate binding protein	-
BN117_RS13060	2733093..2734483	+	1391	Protein_2567	arylsulfatase	-
BN117_RS13065	2734480..2735046	+	567	WP_015039900.1	LamB/YcsF family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T28414 WP_003811545.1 NC_018828:c2730193-2729882 [Bordetella parapertussis Bpp5]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T28414 NC_018828:c2730193-2729882 [Bordetella parapertussis Bpp5]
ATGGAGTTCATCGAAACCCCAATTTTCACGCAATTGATCACCGCGTTGCTGTCGGATGAGGAATATTCCAGGCTGCAACA
GCTGCTGATCGACAACCCCGAAAGGGGCGATCTGCTGCGTGGGGGCGGCGGTATACGAAAGATGCGTTACGGCCGGCAAG
GCACGGGCAAGCGAGGTGGCATCCGGGTCATTTATTACTGGATCAGCCAGGATTGTCAGATCTACATGCTTGCGGCTTAT
GCAAAGTCGAAGAAGATCAACCTGACCCCGGACGAAATCGCGGCGCTGCGCGAACTGGTCAAGGAGCTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT28414 WP_003811547.1 NC_018828:c2729879-2729580 [Bordetella parapertussis Bpp5]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT28414 NC_018828:c2729879-2729580 [Bordetella parapertussis Bpp5]
ATGAAAAAAGACAACACGGTGTTCAACGACCTGCAAGCCAGCCTGAAGCAAGCCGTGGCCATTCGCCAAGGCAAGATGCC
GCCATCGCGTGTATTCAAGGTTGAGACTGTGGATGTGAAGGCAACGCGCGAAAAAACGCACATGTCTCAAACGGAATTCG
CCAAAGCCATCCACGTCAGTGTCCGTACGCTGCAGAACTGGGAGCAAGGTCACCGCAATCCGACGGGCCCTGCCGTGGCG
CTTCTGAAAATCGTGGCAAGCGCCCCGGACATTGCCGTGCGAGCGCTGCGGACCGCGTAA

Structures

Toxin

• AlphaFold DB: K0MEM3