Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3532245..3532915 Replicon chromosome
Accession NZ_CP127277
Organism Mycobacterium tuberculosis strain 25421

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag QRF07_RS16740 Protein ID WP_003899954.1
Coordinates 3532245..3532589 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag QRF07_RS16745 Protein ID WP_003899955.1
Coordinates 3532586..3532915 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF07_RS16705 (QRF07_16705) 3527318..3528178 + 861 WP_003416628.1 alpha/beta hydrolase -
QRF07_RS16710 (QRF07_16710) 3528153..3528668 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
QRF07_RS16715 (QRF07_16715) 3528684..3528895 + 212 Protein_3300 (R)-hydratase -
QRF07_RS16720 (QRF07_16720) 3528908..3529201 + 294 WP_003416635.1 hypothetical protein -
QRF07_RS16725 (QRF07_16725) 3529489..3530778 + 1290 WP_003416640.1 ATP-binding protein -
QRF07_RS16730 (QRF07_16730) 3531125..3531559 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
QRF07_RS16735 (QRF07_16735) 3531562..3532014 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
QRF07_RS16740 (QRF07_16740) 3532245..3532589 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QRF07_RS16745 (QRF07_16745) 3532586..3532915 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
QRF07_RS16750 (QRF07_16750) 3533152..3534413 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QRF07_RS16755 (QRF07_16755) 3534635..3535896 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QRF07_RS16760 (QRF07_16760) 3536169..3536516 + 348 WP_003899956.1 DUF2384 domain-containing protein -
QRF07_RS16765 (QRF07_16765) 3536513..3537133 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T284045 WP_003899954.1 NZ_CP127277:3532245-3532589 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT284045 WP_003899955.1 NZ_CP127277:3532586-3532915 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References