Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3535083..3535753 Replicon chromosome
Accession NZ_CP127276
Organism Mycobacterium tuberculosis strain 5521

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag QRF08_RS16790 Protein ID WP_003899954.1
Coordinates 3535083..3535427 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag QRF08_RS16795 Protein ID WP_003899955.1
Coordinates 3535424..3535753 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF08_RS16755 (QRF08_16755) 3530156..3531016 + 861 WP_003416628.1 alpha/beta hydrolase -
QRF08_RS16760 (QRF08_16760) 3530991..3531506 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
QRF08_RS16765 (QRF08_16765) 3531522..3531733 + 212 Protein_3310 (R)-hydratase -
QRF08_RS16770 (QRF08_16770) 3531746..3532039 + 294 WP_003416635.1 hypothetical protein -
QRF08_RS16775 (QRF08_16775) 3532327..3533616 + 1290 WP_003416640.1 ATP-binding protein -
QRF08_RS16780 (QRF08_16780) 3533963..3534397 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
QRF08_RS16785 (QRF08_16785) 3534400..3534852 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
QRF08_RS16790 (QRF08_16790) 3535083..3535427 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QRF08_RS16795 (QRF08_16795) 3535424..3535753 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
QRF08_RS16800 (QRF08_16800) 3535990..3537251 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QRF08_RS16805 (QRF08_16805) 3537473..3538734 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QRF08_RS16810 (QRF08_16810) 3539007..3539354 + 348 WP_003899956.1 DUF2384 domain-containing protein -
QRF08_RS16815 (QRF08_16815) 3539351..3539971 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T283984 WP_003899954.1 NZ_CP127276:3535083-3535427 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT283984 WP_003899955.1 NZ_CP127276:3535424-3535753 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References