Detailed information of TA system TA028398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 794423..795041
• Replicon: chromosome
• Accession: NC_018700
• Organism: Sinorhizobium meliloti Rm41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: BN406_RS03940
• Protein ID: WP_010969011.1
• Coordinates: 794745..795041 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BN406_RS03935
• Protein ID: WP_010969010.1
• Coordinates: 794423..794731 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN406_RS34300	790238..790474	+	237	WP_153444851.1	hypothetical protein	-
BN406_RS03910	790656..791021	+	366	WP_015007420.1	hypothetical protein	-
BN406_RS37950	792606..792776	-	171	WP_080599795.1	hypothetical protein	-
BN406_RS03920	792869..793843	+	975	WP_051032088.1	non-homologous end-joining DNA ligase	-
BN406_RS03930	794182..794433	-	252	WP_042708639.1	hypothetical protein	-
BN406_RS03935	794423..794731	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
BN406_RS03940	794745..795041	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BN406_RS03945	795089..795394	-	306	WP_003527305.1	hypothetical protein	-
BN406_RS03950	795722..796759	+	1038	WP_003527304.1	porin	-
BN406_RS03955	796869..797936	-	1068	WP_015007424.1	site-specific integrase	-
BN406_RS03960	797977..799026	-	1050	WP_013844229.1	porin	-
BN406_RS03965	799240..799728	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	742239..801938	59699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T28398 WP_010969011.1 NC_018700:c795041-794745 [Sinorhizobium meliloti Rm41]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T28398 NC_018700:c795041-794745 [Sinorhizobium meliloti Rm41]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT28398 WP_010969010.1 NC_018700:c794731-794423 [Sinorhizobium meliloti Rm41]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT28398 NC_018700:c794731-794423 [Sinorhizobium meliloti Rm41]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61