Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3525771..3526441 Replicon chromosome
Accession NZ_CP127275
Organism Mycobacterium tuberculosis strain 6721

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag QRF09_RS16730 Protein ID WP_003899954.1
Coordinates 3525771..3526115 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag QRF09_RS16735 Protein ID WP_003899955.1
Coordinates 3526112..3526441 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF09_RS16695 (QRF09_16695) 3520844..3521704 + 861 WP_003416628.1 alpha/beta hydrolase -
QRF09_RS16700 (QRF09_16700) 3521679..3522194 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
QRF09_RS16705 (QRF09_16705) 3522210..3522421 + 212 Protein_3298 (R)-hydratase -
QRF09_RS16710 (QRF09_16710) 3522434..3522727 + 294 WP_003416635.1 hypothetical protein -
QRF09_RS16715 (QRF09_16715) 3523015..3524304 + 1290 WP_003416640.1 ATP-binding protein -
QRF09_RS16720 (QRF09_16720) 3524651..3525085 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
QRF09_RS16725 (QRF09_16725) 3525088..3525540 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
QRF09_RS16730 (QRF09_16730) 3525771..3526115 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QRF09_RS16735 (QRF09_16735) 3526112..3526441 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
QRF09_RS16740 (QRF09_16740) 3526678..3527939 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QRF09_RS16745 (QRF09_16745) 3528161..3529422 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QRF09_RS16750 (QRF09_16750) 3529695..3530042 + 348 WP_003899956.1 DUF2384 domain-containing protein -
QRF09_RS16755 (QRF09_16755) 3530039..3530659 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T283923 WP_003899954.1 NZ_CP127275:3525771-3526115 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT283923 WP_003899955.1 NZ_CP127275:3526112-3526441 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References