Detailed information of TA system TA028391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3245011..3245764
• Replicon: chromosome
• Accession: NC_018695
• Organism: Burkholderia phenoliruptrix BR3459a

Toxin (Protein)

• Gene name: graT
• Uniprot ID: K0DSG2
• Locus tag: BUPH_RS14070
• Protein ID: WP_015003526.1
• Coordinates: 3245011..3245292 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: K0DUC6
• Locus tag: BUPH_RS14075
• Protein ID: WP_015003527.1
• Coordinates: 3245309..3245764 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BUPH_RS14055	3240524..3241096	+	573	WP_013589301.1	TMEM165/GDT1 family protein	-
BUPH_RS14060	3241143..3241856	-	714	WP_013589302.1	DUF4136 domain-containing protein	-
BUPH_RS14065	3242110..3244815	+	2706	WP_015003525.1	aminopeptidase N	-
BUPH_RS14070	3245011..3245292	+	282	WP_015003526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BUPH_RS14075	3245309..3245764	+	456	WP_015003527.1	HigA family addiction module antidote protein	Antitoxin
BUPH_RS14080	3246012..3247028	+	1017	WP_013589306.1	class 1 fructose-bisphosphatase	-
BUPH_RS14090	3247294..3247479	-	186	WP_175126333.1	hypothetical protein	-
BUPH_RS35840	3248167..3248316	+	150	WP_006050185.1	hypothetical protein	-
BUPH_RS14100	3248594..3249190	+	597	WP_015003529.1	chorismate mutase	-
BUPH_RS14105	3249259..3250419	-	1161	WP_041745694.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (23-99)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10616.23 Da
• Isoelectric Point: 9.0944

>T28391 WP_015003526.1 NC_018695:3245011-3245292 [Paraburkholderia phenoliruptrix BR3459a]
MITTFGDKATARIFQGKFVNTLLRHIQALAYRKLLMIDAAESIHSLHAPPSNRLEALQGQRSGPWSIRVNAQWRICFHFI
DGEALNVEIVDYH

Nucleotide

• Length: 282 bp

>T28391 NC_018695:3245011-3245292 [Paraburkholderia phenoliruptrix BR3459a]
ATGATCACAACATTTGGCGACAAGGCTACGGCAAGAATCTTCCAGGGCAAGTTCGTGAATACGCTGCTGCGTCATATCCA
GGCGCTCGCGTATCGCAAGTTGCTGATGATCGACGCCGCGGAGTCCATCCACAGCTTGCACGCGCCGCCGAGCAACCGGC
TGGAAGCATTGCAGGGTCAGCGCAGCGGACCATGGAGCATCCGGGTCAATGCGCAATGGCGCATCTGTTTTCACTTTATC
GACGGCGAGGCGCTGAATGTTGAGATTGTCGACTATCACTGA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16265.61 Da
• Isoelectric Point: 7.1698

>AT28391 WP_015003527.1 NC_018695:3245309-3245764 [Paraburkholderia phenoliruptrix BR3459a]
MVIKRSDLGSTDFSDIDTGDDIEEIHPGEILRSEFLEPLGMSVNALALALRVPAPRINDVVRGKRAISADTALRLERYFG
ASAQFWLNLQIAYDLRVATAAAGEQIEREIEPMPKADRPKLPKVSAEHARAAALAASLCGSAAAGKARRKG

Nucleotide

• Length: 456 bp

>AT28391 NC_018695:3245309-3245764 [Paraburkholderia phenoliruptrix BR3459a]
ATGGTCATCAAACGCTCCGACCTGGGCAGTACCGATTTCTCCGACATCGACACCGGAGACGACATCGAGGAAATCCATCC
TGGCGAGATTCTGCGCAGCGAGTTCCTCGAACCGCTCGGCATGTCCGTCAATGCGCTCGCGCTTGCGTTGCGCGTGCCGG
CGCCGCGCATCAACGACGTGGTGCGCGGCAAGCGCGCAATCTCCGCCGACACCGCGCTGCGGCTCGAGCGCTACTTCGGC
GCAAGCGCGCAGTTCTGGCTCAATCTGCAAATCGCCTACGACCTGCGCGTTGCCACCGCAGCCGCGGGCGAGCAGATCGA
ACGCGAGATCGAACCCATGCCGAAGGCGGATCGCCCGAAGCTGCCAAAAGTGTCCGCGGAACACGCGCGGGCCGCGGCAC
TTGCGGCAAGCCTTTGCGGCAGCGCGGCGGCCGGCAAGGCGCGGCGCAAGGGCTGA

Structures

Toxin

• AlphaFold DB: K0DSG2

Antitoxin

• AlphaFold DB: K0DUC6