Detailed information of TA system TA028387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2877618..2878275
• Replicon: chromosome
• Accession: NC_018691
• Organism: Alcanivorax dieselolei B5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0CBC7
• Locus tag: B5T_RS12770
• Protein ID: WP_014994925.1
• Coordinates: 2877618..2877971 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: K0CEW1
• Locus tag: B5T_RS12775
• Protein ID: WP_014994926.1
• Coordinates: 2877973..2878275 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5T_RS22725	2872692..2874794	+	2103	WP_167321215.1	Ig-like domain-containing protein	-
B5T_RS23220	2874921..2875412	-	492	WP_148279266.1	hypothetical protein	-
B5T_RS12765	2876029..2877036	-	1008	WP_014994924.1	Rpn family recombination-promoting nuclease/putative transposase	-
B5T_RS22730	2877229..2877369	-	141	WP_081586873.1	DUF4351 domain-containing protein	-
B5T_RS12770	2877618..2877971	+	354	WP_014994925.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B5T_RS12775	2877973..2878275	+	303	WP_014994926.1	XRE family transcriptional regulator	Antitoxin
B5T_RS23525	2878384..2878527	-	144	WP_202803077.1	DUF4351 domain-containing protein	-
B5T_RS12785	2879174..2879407	+	234	WP_014994928.1	transcriptional regulator	-
B5T_RS12790	2879507..2879809	-	303	WP_041717017.1	hypothetical protein	-
B5T_RS12795	2879881..2880156	-	276	WP_014994930.1	transposase	-
B5T_RS12800	2880455..2881147	-	693	WP_014994931.1	transposase	-
B5T_RS12805	2881523..2882344	-	822	WP_202803005.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2869857..2880156	10299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (20-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13445.79 Da
• Isoelectric Point: 10.5969

>T28387 WP_014994925.1 NC_018691:2877618-2877971 [Alcanivorax dieselolei B5]
MWKVQTTDLFDQWFKGRDDSDRVNIIAAMLVLEQKGPYLLRPYADTVKGSVHANMKELRIQSKGRPIRVFFAFDPRRTAV
LLCAGMKAGKEKRFYQTMIPLADKAFSLHLEQLKKGG

Nucleotide

• Length: 354 bp

>T28387 NC_018691:2877618-2877971 [Alcanivorax dieselolei B5]
ATGTGGAAAGTTCAAACAACGGATCTGTTTGACCAGTGGTTCAAAGGGCGTGACGATTCTGATCGGGTAAATATAATCGC
GGCGATGCTTGTTCTGGAGCAGAAAGGACCCTATCTGCTCCGCCCCTACGCGGATACGGTAAAAGGAAGCGTCCACGCCA
ACATGAAGGAACTGCGCATCCAAAGCAAGGGAAGGCCCATCCGGGTGTTTTTCGCCTTCGATCCCCGCAGAACAGCCGTA
CTGCTATGCGCGGGCATGAAAGCCGGCAAGGAAAAACGCTTTTATCAGACGATGATCCCTCTGGCTGACAAAGCGTTCTC
TTTGCATCTGGAGCAACTCAAAAAGGGAGGTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11296.37 Da
• Isoelectric Point: 10.5322

>AT28387 WP_014994926.1 NC_018691:2877973-2878275 [Alcanivorax dieselolei B5]
MGKTLNQILRTEKPEVVRKAKEKADTILLNIHLNEIRTLMRKTQVEMAQSLGVKQPTIAGMEKPGQDLKLSTLKRYIEAA
GGKVRLDIELPDGKHHQIML

Nucleotide

• Length: 303 bp

>AT28387 NC_018691:2877973-2878275 [Alcanivorax dieselolei B5]
ATGGGTAAAACACTGAATCAGATTCTGAGGACCGAAAAGCCCGAGGTCGTTAGAAAGGCAAAGGAGAAGGCCGACACCAT
CCTTCTCAACATCCACCTTAACGAGATCCGCACGCTGATGCGGAAAACACAGGTTGAAATGGCCCAGTCGCTCGGAGTGA
AACAACCCACCATCGCGGGTATGGAAAAGCCCGGACAGGACCTGAAGCTCTCTACTCTGAAGCGCTATATCGAAGCGGCG
GGAGGGAAAGTCCGGCTCGATATAGAGCTACCGGATGGGAAGCACCACCAAATCATGTTGTAG

Structures

Toxin

• AlphaFold DB: K0CBC7

Antitoxin

• AlphaFold DB: K0CEW1