Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3534259..3534929 Replicon chromosome
Accession NZ_CP127274
Organism Mycobacterium tuberculosis strain 17221

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag QRF10_RS16770 Protein ID WP_003899954.1
Coordinates 3534259..3534603 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag QRF10_RS16775 Protein ID WP_003899955.1
Coordinates 3534600..3534929 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QRF10_RS16735 (QRF10_16735) 3529332..3530192 + 861 WP_003416628.1 alpha/beta hydrolase -
QRF10_RS16740 (QRF10_16740) 3530167..3530682 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
QRF10_RS16745 (QRF10_16745) 3530698..3530909 + 212 Protein_3306 (R)-hydratase -
QRF10_RS16750 (QRF10_16750) 3530922..3531215 + 294 WP_003416635.1 hypothetical protein -
QRF10_RS16755 (QRF10_16755) 3531503..3532792 + 1290 WP_003416640.1 ATP-binding protein -
QRF10_RS16760 (QRF10_16760) 3533139..3533573 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
QRF10_RS16765 (QRF10_16765) 3533576..3534028 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
QRF10_RS16770 (QRF10_16770) 3534259..3534603 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QRF10_RS16775 (QRF10_16775) 3534600..3534929 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
QRF10_RS16780 (QRF10_16780) 3535166..3536427 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QRF10_RS16785 (QRF10_16785) 3536649..3537910 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QRF10_RS16790 (QRF10_16790) 3538183..3538530 + 348 WP_003899956.1 DUF2384 domain-containing protein -
QRF10_RS16795 (QRF10_16795) 3538527..3539147 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T283862 WP_003899954.1 NZ_CP127274:3534259-3534603 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT283862 WP_003899955.1 NZ_CP127274:3534600-3534929 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References