Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3533351..3534021 Replicon chromosome
Accession NZ_CP127273
Organism Mycobacterium tuberculosis strain 3320

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag QQ046_RS16745 Protein ID WP_003899954.1
Coordinates 3533351..3533695 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag QQ046_RS16750 Protein ID WP_003899955.1
Coordinates 3533692..3534021 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQ046_RS16710 (QQ046_16710) 3528424..3529284 + 861 WP_003416628.1 alpha/beta hydrolase -
QQ046_RS16715 (QQ046_16715) 3529259..3529774 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
QQ046_RS16720 (QQ046_16720) 3529790..3530001 + 212 Protein_3301 (R)-hydratase -
QQ046_RS16725 (QQ046_16725) 3530014..3530307 + 294 WP_003416635.1 hypothetical protein -
QQ046_RS16730 (QQ046_16730) 3530595..3531884 + 1290 WP_003416640.1 ATP-binding protein -
QQ046_RS16735 (QQ046_16735) 3532231..3532665 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
QQ046_RS16740 (QQ046_16740) 3532668..3533120 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
QQ046_RS16745 (QQ046_16745) 3533351..3533695 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQ046_RS16750 (QQ046_16750) 3533692..3534021 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
QQ046_RS16755 (QQ046_16755) 3534258..3535519 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QQ046_RS16760 (QQ046_16760) 3535741..3537002 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
QQ046_RS16765 (QQ046_16765) 3537275..3537622 + 348 WP_003899956.1 DUF2384 domain-containing protein -
QQ046_RS16770 (QQ046_16770) 3537619..3538239 + 621 WP_003899957.1 RES family NAD+ phosphorylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T283802 WP_003899954.1 NZ_CP127273:3533351-3533695 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT283802 WP_003899955.1 NZ_CP127273:3533692-3534021 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References