Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2249787..2250706 Replicon chromosome
Accession NZ_CP127273
Organism Mycobacterium tuberculosis strain 3320

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag QQ046_RS10575 Protein ID WP_003900449.1
Coordinates 2250101..2250706 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag QQ046_RS10570 Protein ID WP_003410124.1
Coordinates 2249787..2250092 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQ046_RS10540 (QQ046_10540) 2244798..2246054 - 1257 WP_003902247.1 HNH endonuclease signature motif containing protein -
QQ046_RS10545 (QQ046_10545) 2246408..2246983 + 576 WP_003410100.1 hypothetical protein -
QQ046_RS10550 (QQ046_10550) 2246980..2248020 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
QQ046_RS10555 (QQ046_10555) 2248262..2248981 + 720 WP_003410108.1 DUF433 domain-containing protein -
QQ046_RS10560 (QQ046_10560) 2248971..2249387 + 417 WP_003410114.1 hypothetical protein -
QQ046_RS10565 (QQ046_10565) 2249403..2249702 - 300 WP_003410120.1 hypothetical protein -
QQ046_RS10570 (QQ046_10570) 2249787..2250092 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
QQ046_RS10575 (QQ046_10575) 2250101..2250706 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQ046_RS10580 (QQ046_10580) 2250731..2251090 - 360 WP_003410131.1 hypothetical protein -
QQ046_RS10585 (QQ046_10585) 2251250..2251645 - 396 WP_225936727.1 hypothetical protein -
QQ046_RS10590 (QQ046_10590) 2251705..2253222 - 1518 Protein_2093 DEAD/DEAH box helicase family protein -
QQ046_RS10595 (QQ046_10595) 2253732..2254730 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T283784 WP_003900449.1 NZ_CP127273:c2250706-2250101 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT283784 WP_003410124.1 NZ_CP127273:c2250092-2249787 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References