Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3264806..3265359 Replicon chromosome
Accession NZ_CP127255
Organism Escherichia coli strain Q5

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag QQ972_RS15845 Protein ID WP_000605680.1
Coordinates 3265090..3265359 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QQ972_RS15840 Protein ID WP_000781370.1
Coordinates 3264806..3265090 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQ972_RS15825 (3259987) 3259987..3263034 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
QQ972_RS15830 (3263047) 3263047..3263931 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
QQ972_RS15835 (3263924) 3263924..3264577 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
QQ972_RS15840 (3264806) 3264806..3265090 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QQ972_RS15845 (3265090) 3265090..3265359 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQ972_RS15850 (3265545) 3265545..3266555 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
QQ972_RS15855 (3266689) 3266689..3268386 - 1698 WP_000433462.1 malate dehydrogenase -
QQ972_RS15860 (3268543) 3268543..3268680 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QQ972_RS15865 (3268782) 3268782..3268997 - 216 WP_000495766.1 biofilm-dependent modulation protein -
QQ972_RS15870 (3269342) 3269342..3269773 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T283729 WP_000605680.1 NZ_CP127255:c3265359-3265090 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT283729 WP_000781370.1 NZ_CP127255:c3265090-3264806 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References