Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2251234..2251913 Replicon chromosome
Accession NZ_CP127255
Organism Escherichia coli strain Q5

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag QQ972_RS10700 Protein ID WP_000057523.1
Coordinates 2251234..2251536 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag QQ972_RS10705 Protein ID WP_000806442.1
Coordinates 2251572..2251913 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQ972_RS10680 (2246498) 2246498..2247718 - 1221 WP_001251634.1 fosmidomycin MFS transporter -
QQ972_RS10685 (2247936) 2247936..2249588 + 1653 WP_016233905.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
QQ972_RS10690 (2249625) 2249625..2250104 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
QQ972_RS10695 (2250307) 2250307..2251101 - 795 WP_089695977.1 TraB/GumN family protein -
QQ972_RS10700 (2251234) 2251234..2251536 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QQ972_RS10705 (2251572) 2251572..2251913 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
QQ972_RS10710 (2251971) 2251971..2254475 - 2505 WP_000083982.1 copper-exporting P-type ATPase CopA -
QQ972_RS10715 (2254737) 2254737..2255669 + 933 WP_285984985.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T283724 WP_000057523.1 NZ_CP127255:2251234-2251536 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT283724 WP_000806442.1 NZ_CP127255:2251572-2251913 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References