Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 649362..649964 Replicon chromosome
Accession NZ_CP127252
Organism Escherichia coli strain C41

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag QQ971_RS03145 Protein ID WP_000897305.1
Coordinates 649362..649673 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QQ971_RS03150 Protein ID WP_089625183.1
Coordinates 649674..649964 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQ971_RS03120 (645276) 645276..645875 + 600 WP_001295269.1 glucose-1-phosphatase -
QQ971_RS03125 (645869) 645869..646741 + 873 WP_000920762.1 virulence factor BrkB family protein -
QQ971_RS03130 (646738) 646738..647175 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
QQ971_RS03135 (647220) 647220..648161 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
QQ971_RS03140 (648225) 648225..649133 - 909 WP_001162704.1 alpha/beta hydrolase -
QQ971_RS03145 (649362) 649362..649673 + 312 WP_000897305.1 hypothetical protein Toxin
QQ971_RS03150 (649674) 649674..649964 + 291 WP_089625183.1 helix-turn-helix domain-containing protein Antitoxin
QQ971_RS03155 (650550) 650550..650768 + 219 WP_001295676.1 CopG family transcriptional regulator -
QQ971_RS03160 (650986) 650986..651228 + 243 WP_001086388.1 protein YiiF -
QQ971_RS03165 (651457) 651457..652437 - 981 WP_000399648.1 IS110-like element IS621 family transposase -
QQ971_RS03170 (652837) 652837..653766 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
QQ971_RS03175 (653763) 653763..654398 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 651457..652437 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(37-74)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T283687 WP_000897305.1 NZ_CP127252:649362-649673 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10860.45 Da        Isoelectric Point: 10.8907

>AT283687 WP_089625183.1 NZ_CP127252:649674-649964 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISISVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References